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. 2019 Apr;14(4):613–620. doi: 10.4103/1673-5374.248519

Figure 3.

Figure 3

Effects of luteolin on hippocampal neurogenesis in Ts65Dn mice.

(A) Immunofluorescence labeling for the neural stem cell marker nestin (green, FITC) and the astrocyte marker GFAP (red, TRITC) in the hippocampal dentate gyrus. (B) Mean density of nestin and GFAP immunofluorescence. Nestin: genotype (F = 7.47, P < 0.05), treatment (F = 6.14, P < 0.05), genotype × treatment (F = 12.9, P < 0.01). GFAP: genotype (F = 14.1, P < 0.01), treatment (F = 12.1, P < 0.01), genotype × treatment (F = 10.6, P < 0.01). (C) Immunofluorescence for DCX (red, TRITC) and NeuN (green, FITC). (D) DCX+ cell number and NeuN+ cell ratios. DCX: genotype (F = 19.1, P < 0.01), treatment (F = 25.4, P < 0.01), genotype × treatment (F = 16.1, P < 0.01). NeuN: genotype (F = 30, P < 0.01), treatment (F = 22.4, P < 0.01), genotype × treatment (F = 15.7, P < 0.01). Scale bars: 20 μm in A and C. Data are shown as the mean ± SEM (two-way analysis of variance followed by Tukey’s post hoc test). All experiments were performed more than three times. *P < 0.05, **P < 0.01. WT: Wild-type; FITC: fluorescein isothiocyanate; GFAP: glial fibrillary acidic protein; DCX: doublecortin.