Table 1.
Concentrations, masses, and volumes used for calcium phosphate (CP) precipitation.
| Reaction Vessel | Ca(NO3)2 | NTA or EDTA | (NH4)2HPO4 | Solution after Mixing | ||||
|---|---|---|---|---|---|---|---|---|
| c (mM) | V (mL) | c (mM) | m (g) | c (mM) | V (mL) | c(Ca), c(Pi) (mM) | V (mL) | |
| 1 | 40 | 50 | 0 | 0 a,b | - | - | 20 | 100 |
| A | - | - | - | - | 40 | 50 | ||
| 2 | 40 | 50 | 5 | 0.138 a 0.186 b |
- | - | 20 | 100 |
| B | - | - | - | - | 40 | 50 | ||
| 3 | 40 | 50 | 10 | 0.275 a 0.372 b |
- | - | 20 | 100 |
| C | - | - | - | - | 40 | 50 | ||
| 4 | 40 | 50 | 15 | 0.413 a 0.558 b |
- | - | 20 | 100 |
| D | - | - | - | - | 40 | 50 | ||
Numbered solutions (1, 2, 3, 4) and solutions labeled with a letter (A, B, C, D) denote calcium-containing solutions and phosphate solutions, respectively. a Denotes nitrilotriacetic acid (NTA) and b denotes ethylenediaminotetraacetic acid (EDTA) masses in the solutions.