Table 2.

Hit candidate genes from validation screen.

Tier	Gene	Protein	Description
0	DCN	Decorin	Positive Control
1	H2-K1	MHC class I antigen	Strongest hits from validation screen:  • Multiple independent hairpins  • Good reproducibility  • KD effects are consistent within screen an with prior predictions
	Ttk	TTK protein kinase
2	Rgnef	Rho interacting protein 2∣Rho specific exchange factor	• Multiple hairpins  • Effects are consistent within screen and with prior predictions
	Pkp2	Plakophilin 2
	Mlf1	Myeloid leukemia factor 1
3	CD44	CD44 antigen	• Multiple hairpins  • Effects are mostly consistent within screer and with prior prediction
	Ssb	Sjogren syndrome antigen B
	Tsnax	Translin-associated factor X
4	Shc1	src homology 2 domain containing transforming protein 1	•  Multiple hairpins  • Effects are consistent within screen
	Inhba	Activin∣inhibin beta A
	Srsf3	Serine/Arginine-rich splicing factor 3
	Rock2	Rho-associated coiled-coil containing protein kinase 2