Table 4.
Sequence and bacterial diversity estimation of fresh forage and experimental treatment groups.
| Sample ID | Average length (bp) | Observed species | Chao1 | Shannon | Simpson | Goods coverage |
|---|---|---|---|---|---|---|
| FM.1 | 1464 | 1007 | 2741.3 | 8.06 | 0.99 | 0.88 |
| FM.2 | 1469 | 1314 | 4485.7 | 8.13 | 0.99 | 0.88 |
| FM.3 | 1460 | 892 | 1935.4 | 7.59 | 0.99 | 0.92 |
| C90.1 | 1499 | 267 | 437.0 | 1.09 | 0.21 | 1.00 |
| C90.2 | 1501 | 200 | 538.7 | 1.89 | 0.59 | 1.00 |
| C90.3 | 1486 | 350 | 717.1 | 1.49 | 0.40 | 0.99 |
| P90.1 | 1498 | 355 | 701.2 | 3.10 | 0.75 | 0.99 |
| P90.2 | 1500 | 386 | 891.6 | 1.79 | 0.39 | 0.99 |
| P90.3 | 1500 | 493 | 895.2 | 2.45 | 0.57 | 0.99 |
| B90.1 | 1504 | 349 | 640.2 | 2.30 | 0.56 | 0.99 |
| B90.2 | 1504 | 448 | 1103.1 | 2.49 | 0.63 | 0.99 |
| B90.3 | 1490 | 235 | 398.5 | 2.19 | 0.57 | 1.00 |
FM, fresh material; C, control; B, samples treated with L. buchneri; P, samples treated with L. plantarum; samples were fermented for 90 days. Goods coverage is calculated as C = 1 - (s/n), where s is the number of unique CCSs and n is the number of individuals in the sample. This index gives a relative measure of how well the sample represents the larger environment.