Skip to main content
. 2019 Jan 22;10:14. doi: 10.3389/fmicb.2019.00014

FIGURE 4.

FIGURE 4

Impact of PPAP 23 on reactive oxygen species (ROS) generation. (A) Detection of ROS by DCFH2-DA assay. S. aureus HG001 cells were stained with 20 μM DCFH2-DA for 30 min in the dark before the incubation with PPAP 23 for 3 h. Fluorescence Excitation/Emission were measured at 485 nm/535 nm. (B) Enumeration of S. aureus HG001 overnight cultures grown in TSA supplemented with 10 mM ascorbic acid (AA), TSA containing 10 mM AA and 4.6 μM PPAP 23, or TSA with 4.6 μM PPAP 23. (C) Effect of exogenous catalase (1000 U/ml) on the growth of PPAP 23-treated S. aureus HG001. Growth was determined by the optical density in BioTekTM microplate spectrophotometer for 24 h. All data are the mean values of three replicates ± SD. Data of (A) were normalized to the untreated control. Data of (A,B) were analyzed by one-way ANOVA with Dunnett’s posttest, ns, no significant difference, ∗P < 0.05, ∗∗P < 0.01.