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. 2018 Sep 18;7(2):255–274. doi: 10.1016/j.jcmgh.2018.09.006

Figure 8.

Figure 8

IL22 effects on enteroid size and number are resistant to rescue by exogenous wnt. (A) Jejunal enteroids were cultured in WRN without or with IL22 (1 or 5 ng/mL) for 4 days. Representative images are shown. Bar, 500 μm and 200 μm (insets). (B and C) Quantification of cross-sectional area (n = 20–30) and number (n = 3) of jejunal enteroids cultured in WRN with indicated concentrations of IL22. (D) Cldn2 mRNA expression in enteroids (spheroids) cultured in WRN without (blue symbols) or with IL22 (black symbols) (5 ng/mL, PeproTech). *P < .05. (E and F) Ki67 and Pcna expression was markedly reduced in IL22-treated spheroids. Data are representative of at least 3 independent experiments. *P < .05, **P < .01. (G) Jejunal enteroids were cultured in WNR without or with IL22 (5 ng/mL, PeproTech) for 3 days and then pulsed with EdU (red) for 1 hour before fixation. Bar, 25 μm.