Table 1.
Quantitative RT-PCR Primer Sequences
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| Alpi | GGCCATCTAGGACCGGAGA | TGTCCACGTTGTATGTCTTGG |
| Apoa4 | CAACAGGCTGAAGGCTACGAT | CGATTTTTGCGGAGACCTTGG |
| Ascl2 | CTACTCGTCGGAGGAAAG | ACTAGACAGCATGGGTAAG |
| Atoh1 | GAGTGGGCTGAGGTAAAAGAGT | GGTCGGTGCTATCCAGGAG |
| Axin2 | GGACTGGGGAGCCTAAAGGT | AAGGAGGGACTCCATCTACGC |
| Bmi1 | ATCCCCACTTAATGTGTGTCCT | CTTGCTGGTCTCCAAGTAACG |
| Cd3 | ATGCGGTGGAACACTTTCTGG | GCACGTCAACTCTACACTGGT |
| Cd44 | CACCATTGCCTCAACTGTGC | TTGTGGGCTCCTGAGTCTGA |
| ChgA | CCAAGGTGATGAAGTGCGTC | GGTGTCGCAGGATAGAGAGGA |
| ChgB | GCTCAGCTCCAGTGGATAACA | CAGGGGTGATCGTTGGAACAC |
| Cldn2 | GGCTGTTAGGCACATCCAT | TGGCACCAACATAGGAACTC |
| Cmyc | TTCATCTGCGATCCTGACGAC | CACTGAGGGGTCAATGCACTC |
| Dkk1 | TGCATGAGGCACGCTATGTG | GCGGCGTTGTGGTCATTAC |
| Dkk2 | ACCCGCTGCAATAATGGAATC | ATGGTTGCGATCTCTATGCCG |
| Dll1 | GCAGGACCTTCTTTCGCGTAT | AAGGGGAATCGGATGGGGTT |
| Dll4 | TTCCAGGCAACCTTCTCCGA | ACTGCCGCTATTCTTGTCCC |
| F4/80 | TGACTCACCTTGTGGTCCTAA | CTTCCCAGAATCCAGTCTTTCC |
| Fabp1 | ATGAACTTCTCCGGCAAGTACC | CTGACACCCCCTTGATGTCC |
| Fzd7 | CGGGGCCTCAAGGAGAGAA | GTCCCCTAAACCGAGCCAG |
| Gapdh | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |
| Hes1 | TCAACACGACACCGGACAAAC | ATGCCGGGAGCTATCTTTCTT |
| Hopx | ACCACGCTGTGCCTCATCGC | TTCTGACCGCCGCCACTCTG |
| Jagged1 | CCTCGGGTCAGTTTGAGCTG | CCTTGAGGCACACTTTGAAGTA |
| Ki67 | ATCATTGACCGCTCCTTTAGGT | GCTCGCCTTGATGGTTCCT |
| Lgr5 | CCTACTCGAAGACTTACCCAGT | GCATTGGGGTGAATGATAGCA |
| Lrig1 | TTGAGGACTTGACGAATCTGC | CTTGTTGTGCTGCAAAAAGAGAG |
| Lrp5 | AAGGGTGCTGTGTACTGGAC | AGAAGAGAACCTTACGGGACG |
| Lrp6 | TTGTTGCTTTATGCAAACAGACG | GTTCGTTTAATGGCTTCTTCGC |
| Lyz1 | GGAATGGATGGCTACCGTGG | CATGCCACCCATGCTCGAAT |
| Mmp7 | CTGCCACTGTCCCAGGAAG | GGGAGAGTTTTCCAGTCATGG |
| Mpo | AGTTGTGCTGAGCTGTATGGA | CGGCTGCTTGAAGTAAAACAGG |
| Muc2 | ATGCCCACCTCCTCAAAGAC | GTAGTTTCCGTTGGAACAGTGAA |
| Neurog 3 | CCAAGAGCGAGTTGGCACT | CGGGCCATAGAAGCTGTGG |
| Notch1 | GATGGCCTCAATGGGTACAAG | TCGTTGTTGTTGATGTCACAGT |
| Olfm4 | GCCACTTTCCAATTTCAC | GAGCCTCTTCTCATACAC |
| Pcna | TTTGAGGCACGCCTGATCC | GGAGACGTGAGACGAGTCCAT |
| Prom1 | CTCCCATCAGTGGATAGAGAACT | ATACCCCCTTTTGACGAGGCT |
| Prss32 | GCCTGCCATCCTATTTACCTTC | AGTCCAAGTCAATGCTCCTCC |
| Reg3b | ACTCCCTGAAGAATATACCCTCC | CGCTATTGAGCACAGATACGAG |
| Reg3g | ATGCTTCCCCGTATAACCATCA | GGCCATATCTGCATCATACCAG |
| Rnf43 | TCCGAAAGATCAGCAGAACAGA | GGACTGCATTAGCTTCCCTTC |
| Slc5a | ATGCGGCTGACATCTCAGTC | ACCAAGGCGTTCCATTCAAAG |
| Sox9 | GAGCCGGATCTGAAGAGGGA | GCTTGACGTGTGGCTTGTTC |
| Tert | TCTACCGCACTTTGGTTGCC | CAGCACGTTTCTCTCGTTGC |
| Vil1 | TCAAAGGCTCTCTCAACATCAC | AGCAGTCACCATCGAAGAAGC |
| Wif1 | GATCCAACTGTCAATGTCCCTT | ACACGGGAAACCAACTTGAAC |
| Wnt3 | TGGAACTGTACCACCATAGATGAC | ACACCAGCCGAGGCGATG |
| Zpf652 | GGAGCTGGTTGAACCCTGTG | AGGGCTTCCAGACTCCCTTTT |