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. 2019 Jan 23;12:759–771. doi: 10.2147/OTT.S176618

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© 2019 Yin and Ren. This work is published and licensed by Dove Medical Press Limited

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The effect of miR-217 on the chemoresistance of SiHa and Ca-Ski cells.

Notes: (A) The proliferation of SiHa and Ca-Ski cells that were treated with cisplatin were assessed by CCK-8 assay. (B) Effects of different concentrations and time of cisplatin treatment on the level of miR-217 in SiHa and Ca-Ski cells. (C) Effect of cisplatin on mRNA level of KRAS in SiHa and Ca-Ski cells. (D) Effect of cisplatin on the protein expression of KRAS in SiHa and Ca-Ski cells was determined by Western blotting. The experiment was independently repeated three times. (E) The effect of miR-217 down-expression on the sensitivity of cervical cancer cell was assessed by CCK-8. *P<0.05 and **P<0.01 compared to control cell.

Abbreviations: CCK-8, Cell Counting Kit-8; KRAS, Kirsten rat sarcoma viral oncogene homolog; miR-217, microRNA-217; miR-NC, microRNA negative control.