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. 2019 Jan 24;12:825–834. doi: 10.2147/OTT.S192377

Figure 2.

Figure 2

miR-548c-3p targeted HIF1A-AS2 and was negatively regulated by HIF1A-AS2 in MCF-7 cells.

Notes: (A) The target of miR-548c-3p on HIF1A-AS2 3′UTR (wt and mut). (B) The expression level of miR-548c-3p was detected by RT-qPCR in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, #P<0.05 vs miR-548c inhibitor group). (C) The expression level of miR-548c-3p was detected by RT-qPCR in MCF-7 cells transfected with Ad-HIF1A-AS2 or miR-548c mimic and in combination with miR-548c mimic (*P<0.05 vs control, ***P<0.001 vs control, #P<0.05 vs miR-548c mimic group). (D) The activity of HIF1A-AS2 was measured by dual luciferase reporter assay in MCF-7 cells co-transfected with HIF1A-AS2 (wt and mut) and miR-548c mimics (*P<0.05 vs control). “R” represents the relative light unit of Ranilla luciferase. “F” represents the relative light unit of Firefly luciferase.

Abbreviations: HIF1A-AS2, hypoxia-inducible factor-1 alpha antisense RNA-2; mut, mutant; RT-qPCR, quantitative real-time PCR; UTR, untranslated region; wt, wild type.