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. 2019 Jan 24;12:825–834. doi: 10.2147/OTT.S192377

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© 2019 Guo et al. This work is published and licensed by Dove Medical Press Limited

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sh-HIF1A-AS2 suppressed proliferation and senescence by regulating miR-548c-3p in MCF-7 cells.

Notes: (A) The cell proliferation folds were detected by CCK-8 in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, **P<0.01 vs control). (B) The cell senescence was detected by SA-β-gal staining in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, **P<0.01 vs control, ^#P<0.05 vs miR-548c inhibitor group). (C) The expression of proliferation marker proteins Ki-67 and PCNA were measured by Western blot in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor. (D) Histograms display the statistical analysis of Western blotting results. The bars show mean ± SD of three independent experiments (*P<0.05 vs control, ^#P<0.05 vs miR-548c inhibitor group).

Abbreviations: CCK-8, Cell Counting Kit-8; Ctrl, control; HIF1A-AS2, hypoxia-inducible factor-1 alpha antisense RNA-2; SA-β-gal, senescence-associated β-galactosidase.

sh-HIF1A-AS2 suppressed proliferation and senescence by regulating miR-548c-3p in MCF-7 cells.

Notes: (A) The cell proliferation folds were detected by CCK-8 in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, **P<0.01 vs control). (B) The cell senescence was detected by SA-β-gal staining in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, **P<0.01 vs control, ^#P<0.05 vs miR-548c inhibitor group). (C) The expression of proliferation marker proteins Ki-67 and PCNA were measured by Western blot in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor. (D) Histograms display the statistical analysis of Western blotting results. The bars show mean ± SD of three independent experiments (*P<0.05 vs control, ^#P<0.05 vs miR-548c inhibitor group).

Abbreviations: CCK-8, Cell Counting Kit-8; Ctrl, control; HIF1A-AS2, hypoxia-inducible factor-1 alpha antisense RNA-2; SA-β-gal, senescence-associated β-galactosidase.