Figure 4.

sh-HIF1A-AS2 inhibited cell invasion and epithelial–mesenchymal transition (EMT) by regulating miR-548c-3p in MCF-7 cells.

Notes: (A) The invasion ability of MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor was detected by transwell assays. Invasive cells were stained with crystal violet solution, and the quantification of invasive cells is shown (*P<0.05 vs control, ^#P<0.05 vs miR-548c inhibitor group). (B) The expression of EMT marker proteins and VEGF was measured by Western blot in MCF-7 cells transfected with sh-HIF1A-AS2 or miR-548c inhibitor and in combination with miR-548c inhibitor (*P<0.05 vs control, ^#P<0.05 vs miR-548c inhibitor group).

Abbreviations: Ctrl, control; HIF1A-AS2, hypoxia-inducible factor-1 alpha antisense RNA-2; VEGF, vascular endothelial growth factor.