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. 2019 Jan 24;12:825–834. doi: 10.2147/OTT.S192377

Figure 6.

Figure 6

sh-HIF1A-AS2 inhibited tumor growth and motility by targeting miR-548c-3p to regulate HIF-1α/VEGF pathway in vivo.

Notes: (A) Tumor weight was noticeably reduced in sh-HIF1A-AS2 group mice (**P<0.01 vs control). (B) Expression level of miR-548c-3p was detected by RT-qPCR in sh-HIF1A-AS2 group mice (**P<0.01 vs control; ***P<0.001 vs control). (C) Expression of EMT marker proteins and HIF-1α was measured by Western blot in sh-HIF1A-AS2 group mice (*P<0.05 vs control). (D) Expression of proliferation marker proteins Ki-67 and VEGF in formalin-fixed, paraffin-embedded tumors from sh-HIF1A-AS2 group mice was detected by IHC analysis (*P<0.05 vs control). “-” stands for Ctrl group; “+” stands for sh-HIF1A-AS2 group.

Abbreviations: Ctrl, control; EMT, epithelial -mesenchymal transition; HIF-1α, hypoxia-inducible factor-1 alpha; HIF1A-AS2, hypoxia-inducible factor-1 alpha antisense RNA-2; IHC, immunohistochemistry; RT-qPCR, quantitative real-time PCR; VEGF, vascular endothelial growth factor.