Tab.2.

Expression data analyzed by quantitative PCR in PC-3 cells

Atypical chemokine receptors	PWR-1E CT	PC-3 CT	FC	P-value
ACKR1(DARC)	31.95	ND	NA
ACKR2(D6)	33.95	34.95	-4.70	0.0002
ACKR3 (CXCR7)	33.95	29.93	6.86	0.003
ACKR4(CCRL1)	30.95	32.94	-9.34	0.001
ACKR5 (CCRL2)	36.99	24.95	6758.56	0.0001

Note: Fold-change (FC) values were calculated using the 2 ^−ΔΔCT method as described in Materials and methods. For fold changes<1, the negative inverse of the result was reported as a fold decrease. P values were calculated by Student’s t-test (two sided, for triplicate samples) from comparing fold changes in PC-3 cell lines relative to PWR-1E cell line. Expression levels for each target gene were normalized to the expression levels of the reference (housekeeping) genes HPRT1 and β -actin. Gene transcripts with CT (threshold cycles)>37 were considered not detected (ND).