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Proliferation assay. Effect of HLSC (a) and MSC (b) derived EVs at doses of 10E4 or 10E5 particles/cell on HK-2 cells cultured under serum-free conditions. After 72 h, proliferation was determined using a BrdU cell proliferation ELISA. Proliferation of treated samples was expressed as a percentage of the proliferation of untreated cells. * indicates p < 0.05, ** p < 0.01, *** p < 0.001 after one-way ANOVA with Dunnett’s post hoc test.
