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. 2019 Jan 27;34(1):510–518. doi: 10.1080/14756366.2018.1559840

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Diatom cells were spotted on the agar plates containing 0.4 mM of each sulphonamide inhibitors. Panel containing the agar with the inhibitor has been indicated with the acronym of the inhibitor used. The panel C1 and AAZ are separated by the other groups because obtained from a different set of experiments. Legend: C and C1, panels with the plate free of the inhibitor; 10% DMSO, panel with the plate inoculated with 10% DMSO; Panels 1, 2, 3, 4, 5, MZA, AAZ, plates with the sulphonamide inhibitors; Number of cells going from left to right: 2.0 × 10⁷; 1.5 × 10⁷; 1.0 × 10⁷; 0.75 × 10⁷ and 0.5 × 10⁷. Diatom cell survival was monitored after five days under normal light conditions and 18 °C. The disappearance of the diatom colonies evidences the inhibitor effect of the CAIs.