Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 28;26(1):12–22. doi: 10.1080/10717544.2018.1507057

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro. The CCK8 assay, wound-healing assay, and Transwell chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group (p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells (p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.