Figure 5.

SF-EVs promote ESC decidualisation. Primary ESCs (n = 5) were treated with SF-EVs (5 × 10¹⁰ SF-EVs/10⁶ cells) once (day 0 of decidualisation, solid black line) or twice (day 0 and 3 of decidualisation, dashed black line). Cells were treated with PBS in the control (solid grey line). (a) Prolactin concentration in cell supernatants at different SF-EVs exposures. p < 0.05 was considered significant. Prolactin secretion increased significantly after single (**p = 0.0044) and multiple (***p = 0.0021) SF-EV treatments throughout time.