Table 1.
Fluorescence dyes, primary and secondary antibodies used for Western blot, flow cytometry and fluorescence microscopy.
| Antibody/dye | Origin | Concentration/dilution | Antigen | Clones | Source |
|---|---|---|---|---|---|
| Western blotting | |||||
| Anti-ALIX IgG | Mouse | 1/1,000 | ALIX | 3A9 | Cell Signaling Technology, USA |
| Anti- CD9 IgG | Rabbit | 1/1,000 | CD9 | EPR2949 | Abcam®, UK |
| Anti-SYNTENIN-1 IgG | Rabbit | 1/1,000 | SYNTENIN-1 | EPR8102 | Abcam®, UK |
| Anti-TAPA 1 IgG | Rabbit | 1/1,000 | CD81 | Polyclonal | Abcam®, UK |
| Anti-mouse-HRP | Goat | 1/1,000 | Mouse immunoglobulins | Polyclonal | Dako, Denmark |
| Anti-rabbit-HRP | Goat | 1/1,000 | Rabbit immunoglobulins | Polyclonal | Dako, Denmark |
| Flow cytometry/fluorescence microscopy | |||||
| Bio-maleimide (BODIPY FL N-(2-aminoethyl)- maleimide) |
2 µM | Thiol groups | Molecular Probes, USA | ||
| Anti-β actin IgG | Mouse | 1/500 | β-actin | AC-15 | Abcam®, UK |
| Anti-LC3 | Rabbit | 1/500 | LC3 | Polyclonal | MBL International, USA |
| Anti-Mouse IgG (Alexa Fluor® 594nm) | Donkey | 1/200 | Mouse immunoglobulin | Polyclonal | Molecular Probes, USA |
| Anti-Rabbit IgG (Alexa Fluor® 594nm) | Goat | 1/200 | Rabbit immunoglobulin | Polyclonal | Thermo Scientific, USA |
| DAPI | 1/1,000 | Nuclei | Molecular Probes, USA | ||