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Lipid contents of medium-sized EVs measured by the optimised bench top assay and by the IR-based semi-quantitative method. EVs were isolated from 24 mL serum-free conditioned medium (24 h). Mean of three technical replicates ± SD.
| Sample | Protein concentration (ng/µL) | Lipid concentration – new SPVassay (ng/µL) | Lipid concentration – IR method (ng/µL) |
|---|---|---|---|
| mEV H9c2 | 101.7 ± 2.9 | 114.5 ± 3.1 | 144.5 ± 68.6 |
| mEV AC16 | 86.6 ± 2.6 | 105.1 ± 1.5 | 113.9 ± 39.4 |
