Table 3.
Protein and nucleic acid interference with the lipid assay. The presence of proteins or nucleic acids does not affect the optimised lipid assay. Bovine serum albumin (BSA) was used as a test protein and genomic DNA extracted from Saccharomyces cerevisiae cells was used as test nucleic acids. arb: arbitrary unit.
| Absorbance (arb) |
Replicates |
Student t-test |
|||
|---|---|---|---|---|---|
| Protein concentration (µg) | Average | SD | n | p = | One-way ANOVA |
| 0.0 | 0,1839 | 0.0213 | 6 |
F (0.2894) < Fcrit (2.6143) Protein (BSA) concentration significantly do not change the mean absorbance at 540 nm |
|
| 0.63 | 0.1830 | 0.0076 | 3 | 0.9463 | |
| 1.25 | 0.1830 | 0.0176 | 3 | 0.9512 | |
| 2.50 | 0.1953 | 0.0068 | 3 | 0.4076 | |
| 5.00 | 0.1917 | 0.0184 | 3 | 0.6642 | |
| 10.00 | 0.1867 | 0.0101 | 3 | 0.8414 | |
| 20.00 | 0.1933 | 0.0105 | 3 | 0.5020 | |
| 40.00 |
0.1842 |
0.0262 |
3 |
0.9891 |
|
| Absorbance (arb) |
Replicates |
Student t-test |
|||
| DNA concentration (ng) |
n |
p = |
n |
p = |
One-way ANOVA |
| 0.0 | 0.1355 | 0.0178 | 6 |
F (0.3354) < Fcrit (2.5435) DNA concentration significantly do not change the mean absorbance at 540 nm |
|
| 15.6 | 0.1270 | 0.0066 | 3 | 0.4629 | |
| 31.3 | 0.1273 | 0.0176 | 3 | 0.5366 | |
| 62.5 | 0.1323 | 0.0074 | 3 | 0.7822 | |
| 125.0 | 0.1410 | 0.0174 | 3 | 0.6740 | |
| 250.0 | 0.1310 | 0.0017 | 3 | 0.6862 | |
| 500.0 | 0.1313 | 0.0157 | 3 | 0.7428 | |
| 1000.0 | 0.1300 | 0.0121 | 3 | 0.6500 | |