Figure 5.

In vitro testing of N-acetylcysteine (NAC) and P3001 potency on mouse BAL fluid. BAL fluid from wild-type (WT) and βENaC-transgenic mice were subjected to increasing concentration of NAC and P3001 (0.02–20 mM). Samples were incubated at 37°C for 15 minutes and then quenched. (A) Western blotting was used to assess NAC and P3001 potency at different concentrations and at different stoichiometric ratios because mucin concentration was increased fourfold in βENaC mice as compared with WT animals. (B) Reduction of high-molecular-weight multimer intensity that reflects partial of full Muc5b reduction is graphed for NAC (gray circles) and P3001 (black squares) in the two models. NT = no treatment.