Table 2. Overexpressed genes in codY mutant strains.
Overexpression compared to WT | codY mutation | |||
---|---|---|---|---|
Null | F74Y | F101W | E99D | |
>3-fold | 495 | 257 | 173 | 98 |
>5-fold | 333 | 149 | 105 | 39 |
>10-fold | 210 | 91 | 57 | 26 |
>20-fold | 135 | 43 | 40 | 20 |
>50-fold | 71 | 17 | 28 | 15 |
>100-fold | 40 | 7 | 23 | 14 |
As indicated in the first line of data, 495 genes were overexpressed >3-fold in the codY null mutant compared to the WT. Of those 495 genes, 333 were overexpressed >5-fold, 210 were overexpressed >10-fold, etc. Members of the group of genes overexpressed in the null mutant were overexpressed to various extents in the individual point mutants. The ethanolamine gene cluster (CDR20291_1828–1846) has such a low level of expression in the codY null mutant that 13 of the 19 genes were excluded from the listing. However, this gene cluster was very highly overexpressed in the point mutants. The pattern of expression is presented in detailbelow.