Table 3. Hyperexpression of spo genes in a codY null mutant.
| Gene | Expression ratio (codY null/WT) | Gene | Expression ratio (codY null/WT) |
|---|---|---|---|
| spo0A | 4.7 | spoIVA | 304 |
| R20291_1476 (Spo0A kinase) | 3.5 | R20291_0714 (stage IV sporulation protein) |
5.1 |
| spoIIA operon: spoIIAA | 24 | R20291_3400 (spore cortex-lytic enzyme) |
21 |
| spoIIAB | 23 | sspA | 5.7 |
| sigF | 20 | sspB | 4.7 |
| spoIIE | 223 | cspBA | 19 |
| spoIIG operon: spoIIGA | 51 | cspC | 15 |
| sigE | 59 | gerG | 50 |
| spoIIP | 61 | spoVAC | 76 |
| spoIIR | 16 | spoVAD | 21 |
| sigG | 125 | spoVAE | 9.4 |
| spoIIIA operon: spoIIIAA | 995 | spoVD | 60 |
| spoIIIAB | 212 | spoVS | 3.2 |
| spoIIIAC | 177 | spoVT | 670 |
| spoIIIAD | 157 | spore coat assembly | 50 |
| spoIIIAE | 95 | R20291_0212 (spore coat protein) |
7 |
| spoIIIAF | 160 | cotJB1 | 6.5 |
| spoIIIAG | 824 | cotJC1 | 6.6 |
| spoIIIAH | 284 | spmA | 61 |
| spoIIID | 91 | spmB | 14 |
| sleB | 4.6 | cwlD | 4.6 |
| sleC | 3.3 | bclA2 | 5.6 |
| gpr | 384 | bclA3 | 4.1 |
| gerS | 3.8 |
The indicated genes have been shown to be required for spore formation in C. difficile or are homologs of B. subtilis sporulation genes. The expression ratios are based on RPKMO levels as determined by RNA-Seq.