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. 2019 Jan 30;14(1):e0206896. doi: 10.1371/journal.pone.0206896

Table 4. Bacterial strains and plasmids.

Strains and Plasmids Description Source and/or Reference
Plasmids
pJS107 TargeTron vector J. Sorg
pBL100 TargeTron vector [98]
pBL92 pBL100 codY::intron::ermB This work
pBL103 pJS107 codY::intron::ermB This work
pSMB47 E. coli plasmid carrying part of Tn916 [99]
pBL26 Derivative of pSMB47 carrying the cat gene from pJIR1456 [17]
pND3 pBL26::codY+ This work
pND5 pBL26::codY (F74Y) This work
pND6 pBL26::codY (E99D) This work
pND7 pBL26::codY (E103D) This work
pND9 pBL26::codY (F74L) This work
pND11 pBL26::codY (F101W) This work
pND12 pBL26::codY (P102G) This work
Escherichia coli
HB101 (pRK24) F+ supE44 hsdS20(rB+mB+) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 (Tra+ Mob+ Ampr Tcr) [95]
Bacillus subtilis
BS49 Contains integrated Tn916 [100]
Clostridoides difficile
UK1 Ribotype 027 D. Gerding
LB-CD16 UK1 codY::intron::ermB [44]
ND-CD5 UK1 codY::intron::ermB Tn916 This work
ND-CD6 UK1 codY::intron::ermB Tn916::codY (E103D) This work
ND-CD10 UK1 codY::intron::ermB Tn916::codY+ This work
ND-CD12 UK1 codY::intron::ermB Tn916::codY (F74Y) This work
ND-CD13 UK1 codY::intron::ermB Tn916::codY (E99D) This work
ND-CD17 UK1 codY::intron::ermB Tn916::codY (F101W) This work
ND-CD18 UK1 codY::intron::ermB Tn916::codY (P102G) This work