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. 2019 Jan 30;14(1):e0209665. doi: 10.1371/journal.pone.0209665

Fig 3. Vimentin inhibition causes the juxtanuclear clustering of autophagosomes.

Fig 3

A) Immunofluorescence analysis of endogenous vimentin (white), tubulin (red) and GFP-LC3 in HEK293-GFP-LC3 cells treated with 160nM BAF only, 1.5 μM WFA or 1.5 μM WFA & 160nM BAF for 6 h and compared to untreated cells. Cell nuclei were stained with DAPI (blue). Merged panels show the position of GFP-LC3 relative to vimentin, tubulin and nuclei. Scale bars are equal to 10 μm. Representative images are shown. B) Yellow lines define the areas covered by vimentin (panels i-iv) or autophagosomes (panels v-viii). Scale bars are equal 10 μm. Representative images are shown. C) Number of autophagosomes per cell quantified from immunofluorescent images (n = 6 cells). Student’s t-test * p = 0.0144, *** p = 0.0008. D) Vimentin area quantified from immunofluorescent images (n = 6 cells). One-way ANOVA * p ≤0.05. E) Autophagosome area quantified from immunofluorescent images (n = 6 cells). Student’s t-test * p = 0.011.