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. 2019 Jan 30;14(1):e0211623. doi: 10.1371/journal.pone.0211623

Fig 2. Effects of photoperiod and tissue types on the alternative splicing of ZmCCA1 in maize.

Fig 2

The plant seedlings at five fully-expanded leaf stage on either LDs or SDs were harvested at the indicated ZT points of the extraction of total RNA samples. Relative expression level of ZmCCA1.1 (A), ZmCCA1.2 (B), and ZmCCA1.3 (C) in leaves and leaf sheaths were determined by qRT-PCR. At ZT2, the expression of plant seedlings in the leaf sheaths under LD condition, the leaves under LD condition, and the leaves under SD condition were set to 1 in A, B, and C, respectively. LD, long day conditions; SD, short day conditions; Q, leaf sheaths; Y, leave; ZT, Zeitgeber time. Biological triplicates were averaged. Bars indicate the standard error of the mean. Significant differences between normal and drought treatment condition were assessed using Student’s t-test; * P < 0.05.