Table 5. Normfinder rankings (grouped).
All Samples | All Healthy | All Dystrophic | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Animal | Disease | Muscle | Age | Animal | Muscle | Age | Animal | Muscle | Age | |
Best pair |
AP3D1 + HTATSF1 |
HTATSF1 + B2M |
AP3D1 + HTATSF1 |
AP3D1 + CSNK2A2 |
HTATSF1 + CSNK2A2 |
AP3D1 + HTATSF1 |
AP3D1 + HTATSF1 |
PAK1IP1 + RPL13A |
AP3D1 + CSNK2A2 |
AP3D1 + PAK1IP1 |
Most | CSNK2A2 | AP3D1 | CSNK2A2 | ACTB | CSNK2A2 | CSNK2A2 | RPL13A | RPL13A | AP3D1 | RPL13A |
stable | AP3D1 | HPRT1 | AP3D1 | AP3D1 | RPL13A | ACTB | CSNK2A2 | AP3D1 | CSNK2A2 | AP3D1 |
HTATSF1 | CDC40 | ACTB | CSNK2A2 | ACTB | AP3D1 | B2M | CSNK2A2 | ACTB | CDC40 | |
RPL13A | CSNK2A2 | B2M | PAK1IP1 | HTATSF1 | HTATSF1 | ACTB | ACTB | RPL13A | ACTB | |
CDC40 | 18S | HTATSF1 | RPL13A | AP3D1 | RPL13A | AP3D1 | HTATSF1 | HTATSF1 | HTATSF1 | |
PAK1IP1 | FBXW2 | RPL13A | SDHA | B2M | PAK1IP1 | HTATSF1 | PAK1IP1 | HPRT1 | CSNK2A2 | |
ACTB | PAK1IP1 | PAK1IP1 | HTATSF1 | CDC40 | CDC40 | GAPDH | CDC40 | PAK1IP1 | PAK1IP1 | |
B2M | HTATSF1 | 18S | B2M | SDHA | B2M | PAK1IP1 | B2M | B2M | SDHA | |
HPRT1 | B2M | HPRT1 | CDC40 | PAK1IP1 | 18S | FBXW2 | HPRT1 | 18S | B2M | |
GAPDH | RPL13A | FBXW2 | GAPDH | GAPDH | HPRT1 | CDC40 | GAPDH | FBXW2 | GAPDH | |
FBXW2 | GAPDH | CDC40 | FBXW2 | FBXW2 | SDHA | SDHA | SDHA | CDC40 | HPRT1 | |
Least | SDHA | ACTB | SDHA | HPRT1 | 18S | FBXW2 | 18S | 18S | SDHA | 18S |
stable | 18S | SDHA | GAPDH | 18S | HPRT1 | GAPDH | HPRT1 | FBXW2 | GAPDH | FBXW2 |
Normfinder results for the entire dataset or healthy/diseased subsets grouped by different criteria (as indicated: top row; datasets, second row; criterion), ranked from highest scoring (lowest stability value) to lowest scoring (see supplementary data for extended grouped analysis). Grouped analysis also suggests the best pair of genes for normalization (third row), (not necessarily the highest scoring individually). Bold: stability <0.25; italics: stability > 0.4