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. 2019 Jan 30;14(1):e0211384. doi: 10.1371/journal.pone.0211384

Table 5. Normfinder rankings (grouped).

All Samples All Healthy All Dystrophic
Animal Disease Muscle Age Animal Muscle Age Animal Muscle Age
Best pair AP3D1
+
HTATSF1
HTATSF1
+
B2M
AP3D1
+
HTATSF1
AP3D1
+
CSNK2A2
HTATSF1
+
CSNK2A2
AP3D1
+
HTATSF1
AP3D1
+
HTATSF1
PAK1IP1
+
RPL13A
AP3D1
+
CSNK2A2
AP3D1
+
PAK1IP1
Most CSNK2A2 AP3D1 CSNK2A2 ACTB CSNK2A2 CSNK2A2 RPL13A RPL13A AP3D1 RPL13A
stable AP3D1 HPRT1 AP3D1 AP3D1 RPL13A ACTB CSNK2A2 AP3D1 CSNK2A2 AP3D1
HTATSF1 CDC40 ACTB CSNK2A2 ACTB AP3D1 B2M CSNK2A2 ACTB CDC40
RPL13A CSNK2A2 B2M PAK1IP1 HTATSF1 HTATSF1 ACTB ACTB RPL13A ACTB
CDC40 18S HTATSF1 RPL13A AP3D1 RPL13A AP3D1 HTATSF1 HTATSF1 HTATSF1
PAK1IP1 FBXW2 RPL13A SDHA B2M PAK1IP1 HTATSF1 PAK1IP1 HPRT1 CSNK2A2
ACTB PAK1IP1 PAK1IP1 HTATSF1 CDC40 CDC40 GAPDH CDC40 PAK1IP1 PAK1IP1
B2M HTATSF1 18S B2M SDHA B2M PAK1IP1 B2M B2M SDHA
HPRT1 B2M HPRT1 CDC40 PAK1IP1 18S FBXW2 HPRT1 18S B2M
GAPDH RPL13A FBXW2 GAPDH GAPDH HPRT1 CDC40 GAPDH FBXW2 GAPDH
FBXW2 GAPDH CDC40 FBXW2 FBXW2 SDHA SDHA SDHA CDC40 HPRT1
Least SDHA ACTB SDHA HPRT1 18S FBXW2 18S 18S SDHA 18S
stable 18S SDHA GAPDH 18S HPRT1 GAPDH HPRT1 FBXW2 GAPDH FBXW2

Normfinder results for the entire dataset or healthy/diseased subsets grouped by different criteria (as indicated: top row; datasets, second row; criterion), ranked from highest scoring (lowest stability value) to lowest scoring (see supplementary data for extended grouped analysis). Grouped analysis also suggests the best pair of genes for normalization (third row), (not necessarily the highest scoring individually). Bold: stability <0.25; italics: stability > 0.4