Fig 4. The capacity of SmCB1 to induce T_H2, T_H1 and T_H17 cells is dependent on its proteolytic activity.

On day 22, intracellular cytokine/transcription factor staining and flow cytometry were used to determine the frequencies of IL4⁺ GATA3⁺ CD4⁺ T cells (A), IFN-γ⁺ CD4⁺ T cells (B), and IL-17A⁺ RORγt⁺ CD4⁺ T cells (C), in the mesenteric lymph nodes of wild type C57BL/6 mice that received injections of PBS, active SmCB1, or inactivated SmCB1, on days 0, 14 and 21 by the intraperitoneal route. n.s., P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001.