Fig 5. Acute schistosome infection induces a transient T_H17 response in the spleen and mesenteric lymph nodes.

Using intracellular cytokine/transcription factor staining and flow cytometry, the frequency of IL-17A⁺ CD4⁺ T cells (A, B), RORγt⁺ CD4⁺ T cells (C-D), and double-positive IL-17A⁺ RORγt⁺ CD4⁺ T cells (E, F) in the spleens (A, C, E) and mesenteric lymph nodes (B, D, F) of S. mansoni-infected C57BL/6 mice, was determined at 4, 6 and 8 weeks post infection. Non-infected mice were included at each time point, as negative controls. n.s., P > 0.05; *, P < 0.05; ***, P < 0.001.