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. 2018 Apr 27;42(2):132–143. doi: 10.3906/biy-1711-8

Figure 3.

Figure 3

Knockdown efficiency of Crm1 in HNSCC cell lines. Crm1-siRNA and NT-siRNA were transfected in UT-SCC-74A and UT-SCC-74B cells. (a) After 48 h of transfection, the results showed that Crm1-siRNA compared to the NT-siRNA control led to a 66.3% decrease for primary HNSCC cells and 86.5% decrease for metastatic cells in the level of Crm1 mRNA by RT-qPCR (for both P < 0.001) (scale bar, 200 μm). (b) Western blot analysis demonstrated that Crm1 was suppressed significantly at the protein level in Crm1-siRNA compared to the NT-siRNA control in HNSCC cells (for both P < 0.001).