Table 3.
Effect of different concentrations of Pb (0.25 mM, 0.50 mM, and 0.75 mM), EBL (10–7 M), and SA (1 mM) and their interactions on total phenolic and flavonoid content of 30-, 60-, and 90-day-old plants of B. juncea L. *: P < 0.05, **: P < 0.01.
| Treatment | Total phenolic content (mg g–1 of DW) (mean ± SD) | Flavonoid content(mg g–1 of DW) (mean ± SD) | ||||||
|---|---|---|---|---|---|---|---|---|
| Pb | EBL | SA | 30 DAS | 60 DAS | 90 DAS | 30 DAS | 60 DAS | 90 DAS |
| 0 | 0 | 0 | 10.41 ± 0.373 | 12.91 ± 0.151 | 14.32 ± 0.979 | 0.525 ± 0.013 | 0.543 ± 0.085 | 0.722 ± 0.014 |
| 0 | 10–7 M | 0 | 13.63 ± 0.072 | 18.83 ± 0.180 | 18.99 ± 0.355 | 0.812 ± 0.012 | 0.847 ± 0.041 | 1.016 ± 0.076 |
| 0 | 0 | 1 mM | 10.84 ± 0.256 | 16.11 ± 0.284 | 16.99 ± 0.087 | 0.762 ± 0.090 | 0.639 ± 0.010 | 0.908 ± 0.050 |
| 0 | 10–7 M | 1 mM | 18.68 ± 0.219 | 20.89 ± 0.259 | 21.50 ± 0.293 | 1.108 ± 0.018 | 0.561 ± 0.035 | 1.289 ± 0.018 |
| 0.25 mM | 0 | 0 | 11.26 ± 0.155 | 18.16 ± 0.175 | 15.87 ± 0.222 | 0.660 ± 0.02 | 0.726 ± 0.135 | 0.689 ± 0.019 |
| 0.25 mM | 10–7 M | 0 | 16.92 ± 0.154 | 24.58 ± 0.276 | 18.46 ± 0.155 | 0.791 ± 0.009 | 0.900 ± 0.049 | 1.303 ± 0.026 |
| 0.25 mM | 0 | 1 mM | 13.81 ± 0.137 | 20.69 ± 0.615 | 16.76 ± 0.196 | 0.764 ± 0.031 | 0.666 ± 0.019 | 0.914 ± 0.070 |
| 0.25 mM | 10–7 M | 1 mM | 18.17 ± 0.098 | 26.31 ± 0.103 | 21.52 ± 0.123 | 0.919 ± 0.156 | 0.948 ± 0.011 | 1.189 ± 0.010 |
| 0.50 mM | 0 | 0 | 12.43 ± 0.154 | 20.35 ± 0.083 | 17.59 ± 0.231 | 0.893 ± 0.015 | 0.890 ± 0.079 | 0.729 ± 0.062 |
| 0.50 mM | 10–7 M | 0 | 16.35 ± 0.393 | 23.41 ± 0.118 | 21.69 ± 0.193 | 1.075 ± 0.011 | 1.034 ± 0.009 | 1.258 ± 0.023 |
| 0.50 mM | 0 | 1 mM | 13.41 ± 0.221 | 21.99 ± 0.154 | 19.16 ± 0.226 | 0.960 ± 0.046 | 0.923 ± 0.106 | 1.084 ± 0.023 |
| 0.50 mM | 10–7 M | 1 mM | 19.55 ± 0.264 | 26.75 ± 0.143 | 26.35 ± 0.165 | 1.748 ± 0.012 | 1.204 ± 0.016 | 1.513 ± 0.014 |
| 0.75 mM | 0 | 0 | 11.06 ± 0.190 | 23.47 ± 0.196 | 16.50 ± 0.136 | 1.063 ± 0.007 | 0.842 ± 0.084 | 1.009 ± 0.089 |
| 0.75 mM | 10–7 M | 0 | 16.34 ± 0.738 | 28.29 ± 0.122 | 22.88 ± 0.180 | 1.263 ± 0.013 | 1.221 ± 0.050 | 1.533 ± 0.061 |
| 0.75 mM | 0 | 1 mM | 13.50 ± 0.150 | 25.21 ± 0.154 | 20.43 ± 0.262 | 1.141 ± 0.011 | 1.015 ± 0.049 | 1.167 ± 0.029 |
| 0.75 mM | 10–7 M | 1 mM | 19.28 ± 0.190 | 30.36 ± 0.257 | 31.05 ± 0.761 | 1.898 ± 0.622 | 1.318 ± 0.011 | 2.112 ± 0.008 |
| F-ratio(df 3, 32) treatment | 135.4** | 2643.7** | 768.8** | 34.7** | 86.6** | 277.5** | ||
| F-ratio(df 3, 32) dose | 1974.1** | 2019.2** | 2122.1** | 34.5** | 86.4** | 620.6** | ||
| F-ratio(df 9, 32) treatment × dose | 32.27** | 101.27** | 106.39** | 2.43* | 2.64* | 36.62** | ||
| HSD | 0.819 | 0.720 | 0.873 | 0.491 | 0.187 | 0.136 | ||