REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Donkey anti-mouse RRX	Jackson Immuno Research	115-295-003; RRID: AB_2338756
Goat anti-GFP-FITC	Abcam	ab6662; RRID: AB_305635
Goat anti-mouse Alexa-488	Life Technologies	A11029; RRID: AB_138404
Goat anti-rabbit Alexa Fluor-555	Abcam	ab150086; RRID: AB_2722519
Goat anti-rabbit Alexa-488	Life Technologies	A11034; RRID: AB_2576217
Mouse anti-Engrailed	Developmental Studies Hybridoma Bank	4D9; RRID: AB_528224
Rabbit anti-Diaphanous	(Afshar et al., 2000)	N/A
Rabbit Beta-Galactosidase	Life Technologies,	A11132; RRID: AB_221539
Rabbit pMRLC	Cell Signalling	3671S; RRID: AB_330248
Chemicals, Peptides, and Recombinant Proteins
DAPI	Sigma-Aldrich	D8417; RRID: AB_2307445
Fluoromount G slide mounting medium	SouthernBiotech	0100-01
GelPak, 6.5 mil	GelPak	PF-70-x4/6.5mil
Microposit™ EC solvent	Dow Chemicals
Phalloidin Alexa-488	Life technologies	A12379; RRID: AB_2315147
Phalloidin Alexa-555	Life technologies	A34055
Phalloidin Alexa-647	Life Technologies	A22287; RRID: AB_2620155
Su-8 2050	Microchem	Su-8 2050
SYLGARD 184 elastomer kit	Dow Corning	1673921
Y27632 dihydrochloride Rok Inhibitor	Sigma	Y0503
Experimental Models: Drosophila melanogaster
actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry; Arm::GFP	Y. Mao	N/A
actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry; Rho-1::GFP	Y. Mao	N/A
actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry; RokK116a::Venus	Y.Mao from Rokk116a::Venus (Simões et al., 2010)	N/A
actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry; Sqh::GFP	Y. Mao	N/A
actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry; TubGal80ts	Y. Mao	N/A
DEcad::GFP; rnGal4	N. Tapon	N/A
ECad::6XRFP	Gift from Y. Bellaiche	N/A
Ecad::GFP; pnrGal4, Sqh::mCherry	Ecad::GFP knock-in (Huang et al., 2009). Gift from B. Baum	N/A
Ecad::tomato	(Huang et al., 2009)	N/A
mRFP.nls,w,hsF,Frt19A	Bloomington Drosophila Stock Centre	#31418
Rho-1::GFP	Kyoto Stock Centre	DGRC ID: 110833
Sqh::GFP, enGal4	Y. Mao	N/A
Sqh::GFP, enGal4; UAS-Act5C::RFP	Y. Mao	N/A
Sqh::mCherry	E. Wieschaus	N/A
SqhAx3;;Sqh::GFP	Jordan and Karess, (1997)	N/A
UAS-Act5C::RFP	K. Roper (Roper et al., 2005)	N/A
UAS-AIP1RNAi	Vienna Drosophila RNAi Centre	VDRC, ID: 22851
UAS-arp2RNAi	Vienna Drosophila RNAi Centre Fly stocks of National Institute of Genetics	VDRC:KK10199 NIG, ID: 9901-R
UAS-Cd8::GFP, nubGal4	Mao et al. (2013)	N/A
UAS-cofilinRNAi	Vienna Drosophila RNAi Centre	VDRC, ID: 110599
UAS-DiaphanousRNAi	Vienna Drosophila RNAi Centre	VDRC:KK103914 20518 GD
UAS-drakRNAi	Vienna Drosophila RNAi Centre	VDRC, ID:107263
UAS-e-cadherinRNAi	Vienna Drosophila RNAi Centre	VDRC:KK103962
UAS-enabledRNAi	Vienna Drosophila RNAi Centre	VDRC, ID: 43058
UAS-mlckRNAi	Vienna Drosophila RNAi Centre	VDRC, ID:109937KK
UAS-paxillinRNAi	Vienna Drosophila RNAi Centre	VDRC, ID:107789
UAS-pebbleRNAi	A. Mueller	N/A
UAS-rho kinaseRNAi	Vienna Drosophila RNAi Centre	VDRC:KK104675
UAS-rho RNAi	Vienna Drosophila RNAi Centre	VDRC, ID:109420 KK
UAS-spaghetti squashRNAi	Fly stocks of National Institute of Genetics	NIG, ID: HMS00830 and HMS00437
UAS-Sqh[AA]/TM6B	B. Baum	N/A
UAS-src42ARNAi	N. Tapon	N/A
UAS-src64BRNAi	N. Tapon	N/A
UAS-vinculinRNAi	Vienna Drosophila RNAi Centre	VDRC, ID: 34586
UAS-zyxinRNAi	Fly stocks of National Institute of Genetics	NIG ID: 32018R-1 and 32018R-3
UAS-α-actininRNAi	Vienna Drosophila RNAi Centre	VDRC, ID:107263
UAS–torsoD/βcyt	Martin- Bermudo and Brown, (1999)	N/A
Ubi-dia::GFP	P. Gaspar and N. Tapon	N/A
ubi-dia::GFP; actin-FRT-stop-FRT-Gal4-UAS-Cd8::mCherry/CyO	Y. Mao	N/A
ubi-Ecad::GFP; hhGal4, UAS-IAP/TM6; Sqh::mCherry	(II) - N. Tapon (III) - (Martin et al., 2009)	N/A
ubi-mRFP.nls,w,hs.flp,Frt19A;; Arm-GFP	Y. Mao	N/A
ubi-mRFP.nls,w,hs.flp,Frt19A;; Sqh-GFP	Y. Mao	N/A
yw hsflp; dia iR	Y. Mao from VDRC:103914 KK	N/A
yw hsflp; rho iR	Y. Mao from VDRC:109420/KK	N/A
yw hsflp; rok iR	Y. Mao from VDRC:KK104675	N/A
yw hsflp; UAS-zip iR/SM6-TM6	Y. Mao	N/A
yw hsflp; zip iR; Arm::GFP/SM6-TM6	Y. Mao	N/A
yw,Drok2,Frt19A/FM7c	Bloomington Drosophila Stock Centre	#6666
yw; act-FRT-stop-FRT-Gal4-UASlacZ/ CyO	Y. Mao	N/A
Software and Algorithms
Epitools	Heller et al. (2016)https://epitools.github.io/wiki/	N/A
GraphPad Prism 7	GraphPad Software	N/A
ImageJ 1.51w	Schneider et al. (2012)https://imagej.nih.gov/ij/docs/install/osx.html	N/A
Vertex model	Bespoke model code developed in C++, as in Mao et al. (2013).	N/A