Representative SubAP-MALDI-Orbitrap mass spectrum of peptide standard mixture. The peptides were individually dissolved in water and then combined with matrix to the final amounts: FMRFamide, 71 nmol; FMRF-like neuropeptide, 0.019 nmol; somatostatin-14, 71 nmol; Bradykinin, 0.022 nmol; Aβ (1–16), 0.014 nmol and human insulin, 0.001 nmol. CHCA was taken as MALDI matrix in 10 mg/mL concentration. Laser fluence was 9.6% (100%, 2 μJ) and laser repetition rate was 1 kHz. Source pressure was kept at 3 Torr.