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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Mol Neurosci. 2018 Jul 30;68(3):439–451. doi: 10.1007/s12031-018-1137-8

Fig. 4.

Fig. 4

Conditional knockdown of PAC1 in sympathetic ganglia alters CD4+ helper T-cell polarization during EAE. Cell suspensions from (a, top portion) spleen and (b) lymph nodes were stained with antibodies against CD4, IFNγ, IL-4, and IL-17 and analyzed by flow cytometry to characterize the populations of Th1, Th2, and Th17 CD4+ helper T-cells. Naïve floxed=5, EAE floxed=14, EAE cKD=9. RNA was extracted from whole lymph nodes (data not shown) and spleen (a, bottom portion), and analyzed by qPCR for signature transcription factors and cytokines associated with Th1, Th2, and Th17 CD4+ T-cells. Naïve floxed=4, EAE floxed=5, EAE cKD=6. *p<0.05, **<0.01. Statistical significance was determined by 2-tailed t-tests. Standard error bars are shown