Effect of radiofrequency ablation on recovered RRel spectra. Representative NIR spectral measurements are shown for progressive RF treatment within RV (a), RA (b), and LA (c) tissues. Spectra were normalized to a value of one at 960nm for emphasis on spectral shape differences. (d)-(f) depicts mean +/− 95% confidence interval for normal (n=6) and moderately (n=6) treated tissue for RV, RA, and LA tissues, respectively. Lesions >= 5mm in RV tissue were selected while the criterion for moderate treatment was transmurality (2–4mm) in LA and RA samples. Characteristic differences between untreated and treated spectral shapes were used to guide the calculation of lesion optical indices.