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. 2018 Feb 28;16(2):513–518. doi: 10.1016/j.jgeb.2018.02.009

Fig. 5.

Fig. 5

RFLP patterns of BoLA-DRB3 gene digested by HaeIII restriction enzyme. M: 50-bp ladder. Lanes 1 and 2: AA genotype with two digested fragments at 167- and 135-bp. Lanes 3 and 4: AB genotype with five digested fragments at 190-, 167-, 135-, 83- and 29-bp. Lanes 5 and 6: AC genotype with five digested fragments at 167-, 164-, 135-, 83- and 55-bp. Lanes 7 and 8: DD genotype with four digested fragments at 167-, 83-, 48- and 4-bp. Lanes 9 and 10; CE genotype with four digested fragments at 219-, 164-, 83- and 55-bp. The faint bands at 302-bp are residues from the non-digested original band. The closely bands with sizes at 167- and 164-bp showed as one band on agarose gel. The bands at 55- and 48-bp appeared as one band with the primer dimer. The very small bands at 29- and 4-bp could not be shown on the agarose gel.