Table 2.
Effect of incubation time, temperature, initial pH, and moisture content on xylanase production by S. commune ARC-11.
| Cultural Parameter | Xylanase activity (IU/gds) | Protein content (mg/ml) | SSF conditions | |
|---|---|---|---|---|
| Incubation time (days) | 2 | 256.00 ± 7.99 | 0.85 ± 0.03 | 1. Incubation time-varied, 2. Temperature-30 °C, 3. Initial moisture level-77.5%, 4. Initial pH-5.0 |
| 3 | 338.93 ± 10.85 | 0.97 ± 0.04 | ||
| 4 | 1389.07 ± 35.42 | 1.11 ± 0.03 | ||
| 5 | 1490.93 ± 40.85 | 1.44 ± 0.05 | ||
| 6 | 4262.76 ± 153.46 | 1.69 ± 0.06 | ||
| 7 | 4556.98 ± 212.36 | 1.73 ± 0.06 | ||
| 8 | 5199.02 ± 132.06 | 1.86 ± 0.08 | ||
| 9 | 4039.91 ± 148.67 | 1.99 ± 0.06 | ||
| 10 | 2898.93 ± 93.93 | 1.96 ± 0.08 | ||
| 11 | 1747.73 ± 44.39 | 1.95 ± 0.07 | ||
| 12 | 1472.80 ± 33.87 | 1.90 ± 0.06 | ||
| Temperature (°C) | 26 | 4001.96 ± 158.08 | 1.53 ± 0.09 | 1. Incubation time-8 days, 2. Temperature-varied, 3. Initial moisture level-77.5%, 4. Initial pH-5.0 |
| 30 | 5358.93 ± 253.48 | 1.88 ± 0.09 | ||
| 34 | 3689.87 ± 143.17 | 1.58 ± 0.07 | ||
| 38 | 1829.07 ± 88.89 | 1.50 ± 0.08 | ||
| 42 | ND | ND | ||
| Initial pH | 4.0 | 5242.40 ± 144.69 | 1.65 ± 0.09 | 1. Incubation time-8 days, 2. Temperature-30 °C, 3. Initial moisture level-77.5%, 4. Initial pH-Varied |
| 5.0 | 5299.73 ± 171.18 | 1.92 ± 0.09 | ||
| 5.5 | 5459.73 ± 191.09 | 1.98 ± 0.12 | ||
| 6.0 | 5661.16 ± 157.38 | 2.17 ± 0.11 | ||
| 6.5 | 5973.33 ± 221.01 | 2.24 ± 0.10 | ||
| 7.0 | 6340.71 ± 207.34 | 2.25 ± 0.12 | ||
| 8.0 | 5424.18 ± 227.82 | 2.12 ± 0.11 | ||
| 9.0 | 4934.31 ± 188.49 | 1.94 ± 0.10 | ||
| 10.0 | 4811.82 ± 188.62 | 1.90 ± 0.12 | ||
| Initial moisture content (%) | 55.0 | 3026.13 ± 86.24 | 1.32 ± 0.07 | 1. Incubation time-8 days, 2. Temperature-30 °C, 3. Initial moisture level- 70.0%, 4. Initial pH-7.0 |
| 60.0 | 4118.49 ± 130.14 | 1.56 ± 0.08 | ||
| 65.0 | 5459.73 ± 177.44 | 1.83 ± 0.10 | ||
| 70.0 | 6721.96 ± 235.27 | 2.14 ± 0.10 | ||
| 75.0 | 6262.67 ± 180.36 | 2.30 ± 0.12 | ||
| 77.5 | 4428.62 ± 217.00 | 2.21 ± 0.12 | ||
| 80.0 | 2054.31 ± 87.51 | 2.15 ± 0.11 | ||
| 82.5 | 1619.73 ± 51.83 | 1.92 ± 0.10 | ||
| 85.0 | 1303.64 ± 49.80 | 1.87 ± 0.09 | ||
± Refers standard deviation.