Fig. 1.

Micropropagation of Adhatoda vasica: (a) Direct shoot initiation (arrows) from nodal segment explant after one week of inoculation in MS medium with 1.1 mg/l BA, (b) elongation and proliferation of initiated shoots at 10 days of culture, (c) multiple shoot proliferation and root initiation (arrows) in MS medium with 1 mg/l IBA and 0.25 mg/l NAA, (d) in vitro rooting in clump after 14 days of inoculation (arrows), (e) induction of friable calli in MS medium with 3 mg/l 2,4-D, (f) induction of organogenic calli in MS medium with 0.5 mg/l 2,4-D, (g) indirect rooting and shoot bud (arrows) initiation after 21 days. [Photographs are not in scale].

Source: Original and unpublished photographs from the experiments, carried out by the authors.