Table 2.

Physicochemical properties of Nif A protein (M. wt.: Molecular weight; pI: Isoelectric point; −R: Number of negative residues; +R: Number of positive residues; EC: Extinction coefficient at 280 nm; II: Instability index; AI: Aliphatic index; GRAVY: Grand Average Hydropathy).

S.No	Name of the organism	M. Wt.	Seq. length	pI	EC (assuming all pairs of Cys residues form cystine)	EC (assuming all Cys residues are reduced)	Half Life (hrs)	II	GRAVY	−R	+R	AI
1	B. japonicum	38383.33	353	9.30	18,825	18,450	20	45.32	−0.085	39	48	95.92
2	R. leguminosarum	36926.28	343	8.96	10,470	9970	20	30.65	−0.121	38	45	95.10
3	M. Ciceri	38765.72	352	9.13	17,460	16,960	30	37.40	−0.261	43	52	90.43