FIGURE 5.

Ethanol withdrawal increases the expression of the GluA1 AMPA subunit and of mGluR5 proteins in organotypic hippocampal slices. Experiments were conducted as described in Figure 1A. At the end of the experiments hippocampal slices were lysed and processed for Western blot. Top: representative Western blots using antibodies against the post-synaptic proteins PSD95, GluN1, GluN2A, GluN2B, GluA1, GluA2, mGluR1, and mGluR5 (A–H). The numbers on the left indicate the position of the molecular mass markers (kDa). Tubulin was used as loading control. Bottom: quantitative analysis of immunoreactive bands, showing that ethanol withdrawal increases the expression of the AMPA subunit GluA1 and of metabotropic glutamate receptors mGluR5. Data are expressed as percentage of control. Bars represent the mean ± SEM of at least three experiments. ^∗P < 0.05 vs. CRL (ANOVA + Dunnet’s test).