Table 1.
Primers used for the quantification of the mRNA expression by real-time PCR.
| Target Gene | Gene Symbol | GenBank Accession no. | Position | Product length (bp) | Sequence of Primers |
|---|---|---|---|---|---|
| Agouti-related peptide | agrp | XM_009303347 | 295–443 | 148 | (F) ATGAGGATCTGGGCAAAGCTGT |
| (R) GAAGGCCTTAAAGAAGCGGCAGTA | |||||
| Cocaine and amphetamine-regulated transcript | cartpt1 | AW280905.1 | 1–75 | 75 | (F) TAGACACGATGGTCGGAGAGC |
| (R) CACAGCGAACCAGCAGCATT | |||||
| Elongation factor 1 alpha | ef1-α | NM_131263.1 | 1414–1516 | 103 | (F) GTGCTGGCAAGGTCACAAAG |
| (R) AGAGGTTGGGAAGAACACGC | |||||
| Glucokinase | gck | NM_001045385.2 | 952–1119 | 168 | (F) ACGAGAAGCTGATTGGTGGG |
| (R) TGTCCCCTGTGTCACTCTCA | |||||
| Ghrelin | ghrl | NM_001083872.1 | 23–158 | 136 | (F) GCAGCATGTTTCTGCTCCTG |
| (R) TCAGCAGCTTCTCTTCTGCC | |||||
| Ghrelin O-acyltransferase | mboat4 | NM_001122944.1 | 988–1230 | 242 | (F) CCTGGGCAGATTCTGGGTTT |
| (R) CAGAGCACTCAGCAGTGGAA | |||||
| Growth hormone secretagogue receptor | ghsr | NM_001146272.1 | 439–589 | 151 | (F) GTAGTCGTGACCAAGGGTCG |
| (R) ATTCCGTCGCTTTGCATTCG | |||||
| neuropeptide Y | npy | NM_131074 | 360–551 | 191 | (F) CTGTGATGTCCATGTGTCCTTCTG |
| (R) GAGCCTAAAGAGCGCACATTGA | |||||
| Pro-opiomelanocortin | pomc | NM_131074 | 220–383 | 163 | (F) CAGAGTCTGAGCTTGGGTTTGCTT |
| (R) ACTTTTACCGGTCTGCGTTTGC |