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The apparent kinetic parameters of the transamination reaction catalyzed by AF0933.
| Substrate | Vmax (U/mg) | Km (mM) | kcat/Km (s−1 mM−1) |
|---|---|---|---|
| 3-methyl-2-oxobutyratea | 6.3 | 0.42 | 8.85 |
| 4-methyl-2-oxopentanoatea | 5.5 | 0.25 | 12.98 |
| 3-methyl-2-oxopentanoatea | 3.9 | 0.13 | 17.7 |
| L-glutamateb | 64.6* | 121 | 0.32 |
At 10 mM L-glutamate.
At 5 mM 3-methyl-2-oxobutyrate.
The Vmax for L-glutamate is an extrapolated value calculated at saturated concentration of co-substrate.
