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. 2019 Jan 24;13:8. doi: 10.3389/fncel.2019.00008

FIGURE 4.

FIGURE 4

Comparison of the expression efficacy in SCs of the cochlea between AAV vectors with different promoters. (A) Representative confocal images of transduction of AAV2/2-CBA, AAV2/2-CMV-beta-Globin, AAV2/9-CBA and AAV2/9-CMV-beta-Globin in targeting SCs of the apical and middle turns of the cochlea in vitro. There were many Sox2+/eGFP+ SCs (arrows) in the apical and middle turns of the cochlea when transduced with AAV2/9-CBA. (B) Comparison of the expression efficiencies between AAV2/2-CBA and AAV2/2-CMV-beta-Globin in SCs of the apical, middle, and basal turns of the cochlea in vitro at a working titer of 1 × 1012 VG/ml. AAV2/2-CBA had greater expression efficacy in SCs than AAV2/2-CMV-beta-Globin on average, although there was no statistically significant difference between the expression efficiencies of AAV2/2-CBA and AAV2/2-CMV-beta-Globin in the apical, middle, or basal turn, respectively. (C) Comparison of the expression efficiencies between AAV2/9-CBA and AAV2/9-CMV-beta-Globin in SCs of the apical, middle, and basal turns of the cochlea in vitro at a working titer of 1 × 1012 VG/ml. AAV2/9-CBA had greater expression efficacy in SCs than AAV2/9-CMV-beta-Globin on average. n = 4. Scale bar: 20 μm.