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. 2019 Jan 24;13:8. doi: 10.3389/fncel.2019.00008

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Copyright © 2019 Gu, Chai, Guo, Dong, Li, Shu, Huang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The confocal images of AAV2/Anc80L65-CMV-beta-Globin transduction into the cochlea after in vivo injection and comparison of the in vivo transduction efficiencies in IHCs, OHCs, and SCs between AAV2/9-CBA and AAV2/Anc80L65-CMV-beta-Globin. (A) Transduction of AAV2/Anc80L65-CMV-beta-Globin in HCs of the apical, middle, and basal turns of the cochlea after in vivo injection. (B) Transduction of AAV2/Anc80L65-CMV-beta-Globin in SCs of the apical, middle, and basal turns of the cochlea after in vivo injection. The red arrows indicate the Sox2⁺/eGFP⁺ SCs. (C) Comparison of the in vivo transduction efficiencies in IHCs between AAV2/9-CBA and AAV2/Anc80L65-CMV-beta-Globin. (D) Comparison of the in vivo transduction efficiencies in OHCs between AAV2/9-CBA and AAV2/Anc80L65-CMV-beta-Globin. Overall, the in vivo transduction efficiency of AAV2/Anc80L65-CMV-beta-Globin in IHCs and OHCs was statistically significantly greater than the transduction efficiency of AAV2/9-CBA. (E) Comparison of the in vivo transduction efficiencies in SCs between AAV2/9-CBA and AAV2/Anc80L65-CMV-beta-Globin. AAV2/Anc80L65-CMV-beta-Globin induced more SCs to express eGFP than AAV2/9-CBA on average (24.33 ± 1.83% vs. 17.87 ± 1.88%, p = 0.0130). ^∗p < 0.0167, ^∗∗p < 0.0033, n = 3. Scale bar: 20 μm.