Table 1.
The transduction efficiencies of AAV2/2-CBA and AAV2/9-CBA in IHCs, OHCs, and SCs in the conditions of different viral titers.
| Serotype | Cell type | Viral titer (VG/ml) | Apex (%) | Mid (%) | Base (%) |
|---|---|---|---|---|---|
| AAV2/2 | IHCs | 1 × 1011 | 6.0 ± 3.2 | 3.9 ± 2.5 | 11.7 ± 12.7 |
| 1 × 1012 | 7.3 ± 5.8 | 9.1 ± 10.4 | 3.6 ± 2.1 | ||
| OHCs | 1 × 1011 | 35.1 ± 3.3 | 47.8 ± 16.4 | 68.2 ± 17.7 | |
| 1 × 1012 | 56.2 ± 18.0 | 62.1 ± 21.7 | 75.2 ± 9.2 | ||
| SCs | 1 × 1011 | 35.8 ± 10.6 | 6.9 ± 9.1 | 4.2 ± 4.1 | |
| 5 × 1011 | 47.1 ± 7.4 | 32.5 ± 30.6 | 24.6 ± 24.7 | ||
| 1 × 1012 | 61.4 ± 21.9 | 40.8 ± 38.3 | 7.6 ± 1.4 | ||
| AAV2/9 | IHCs | 1 × 1011 | 6.9 ± 2.9 | 4.2 ± 0.9 | 6.6 ± 1.7 |
| 1 × 1012 | 39.0 ± 17.4 | 41.6 ± 11.0 | 40.1 ± 14.9 | ||
| OHCs | 1 × 1011 | 4.5 ± 1.1 | 3.0 ± 1.9 | 26.2 ± 5.9 | |
| 1 × 1012 | 25.9 ± 7.0 | 30.5 ± 12.2 | 17.9 ± 6.2 | ||
| SCs | 1 × 1011 | 4.3 ± 5.1 | 0 | 5.5 ± 11.1 | |
| 5 × 1011 | 10.2 ± 11.8 | 19.1 ± 16.4 | 4.3 ± 5.2 | ||
| 1 × 1012 | 65.1 ± 20.6 | 53.6 ± 15.1 | 39.1 ± 11.8 |
The values were represented as mean ± standard deviation (n = 5 for HCs; n = 4 for SCs).