Table 1.

The specific primers and probes designed for M. ovipneumoniae detection

Name of primers	Type	Sequences (5′→3′)	Sizes of amplicons (bp)
PCR
EF-TUF	Forward prime	ATGGCAGTTGTTAAAACTGGTG	1209
EF-TUR	Backward primer	TTATTTAATAATTTCAGTTACTGTTCC
LAMP
EF-TUF3	Forward-outer prime	AAAACAGTTGTAACCGGAATT	211
EF-TUB3	Backward-outer primer	ATGGAGTATGTCTTCCACC
EF-TUFIPa (F1c + TTTT + F2)	Forward-inner primer	Biotin -TACGGTCAACACCACGAAGAAGTTTTGTTTAACAAAAACCTTCAATCTGC	165
EF-TUBIP (B1c + TTTT + B2)	Backward-inner primer	GGCAAGTTATTGCAAAACCAAAAACTTTTTCTTCTTTTTTAAGCGCGTAA
LB	Loop-backward primer	TTCCCCACACTAAATTTAAAGCAGC	117
LF	Loop-forward primer	CGGCATTATCTCCGGCCATT
Probe for LAMP-LFD EF-TU-HPb	Hybridization probe	Digoxin -GTTCTTCTTCGTGGTGTTGA	/

^a5′-Labeled with biotin when used in the LAMP-LFD assay

^b5′-Labeled with digoxin when used in the LAMP-LFD assay