Fig. 6.

ETV7 enhances cell proliferation of BM-derived myeloid cells after long-term culture. a MethoCult assays were performed using total BM cells of 8-week-old WT and ETV7Tg mice. Colonies in the first plating (MC1) were counted at 10–12 days post seeding, followed by pooling and replating in new MethoCult medium for an additional 3 rounds (MC2–MC4). *p < 0.05. b Cell growth was examined of WT and ETV7Tg lineage-depleted (Lin⁻) BM cells in liquid culture in the presence of myeloid cytokines from Day 4–9 post seeding. *p < 0.05, **p < 0.01. c Lin⁻ BM cells cultured for 6 days were stained with the indicated antibodies and subjected to FCM analysis. d Cell cycle status was analyzed by flow cytometry at Day 19 of in vitro culture and the bar graph shows the proportion of cells in G₁/G₀, S, and G₂/M phase of the cell cycle *p < 0.05. e The percentage of cells undergoing apoptosis at Day 19 of culture was determined by FCM analysis following AnnexinV/DAPI staining