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. 2019 Jan 24;9:1568. doi: 10.3389/fphar.2018.01568

FIGURE 2.

FIGURE 2

Differential kinetic behaviors of R27T and IFN-β-1a. Kinetic analysis of R27T (left panels) and IFN-β-1a (right panels) binding to AR2Fc (A), AR1Fc (B), and AR1/2Fc (C) chimeric proteins using a BIAcore T200 SPR instrument with 11 (top, 0.097–100 nM), seven (middle, 6.25–400 nM), and 12 different concentrations (bottom, 0.097–200 nM), and a CM5 gold chip with captured anti-human Fc IgG (see the section “Materials and Methods”). Association (Ka) and dissociation (Kd) constants were determined using the 1:1 Langmuir model (the right panel of A), two-state binding model (the left panel of A, B), and heterogeneous ligand model (C) as indicated by the black lines. (D) Normalized response units from sensorgrams of 100 nM R27T and IFN-β-1a with each receptor (AR2Fc, red; AR1Fc, cyan; AR1/2Fc, blue). Kinetic constants of the binding of each ligand to each receptor are listed in Tables 1, 2.