Fig. 2.
SDS-PAGE (A) and western blot analysis (B) of the expression of pGEX-2T or pGRK32 in E. coli. Lane M: Prestained protein marker, Lane 1: E. coli containing pGEX-2T before IPTG induction, Lane 2: bacteria containing pGEX-2T after IPTG induction, Lane 3: bacteria containing pGRK32 before IPTG induction, Lane 4: bacteria containing pGRK32 after IPTG induction.