Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 30;9:951. doi: 10.1038/s41598-018-37264-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

EWS-FLI1 ubiquitination is modulated by USP19. (a) EWS-FLI1 interacts with USP19. 3xflag-EWS-FLI1 and 3xmyc-USP19 were co-expressed in HEK293T cells for 48 h. After co-immunoprecipitation, lysates were analyzed by western blotting as indicated. (b) USP19 depletion increases EWS-FLI1 ubiquitination. A673 cells were transiently incubated with three different siRNAs against USP19 or a control siRNA for 24 h followed by co-expression of 3xflag-EWS-FLI1 and HA-ubiquitin for 48 h. Ubiquitination of EWS-FLI1 was analyzed by western blot analysis using anti-HA antibody. (c) EWSR1 also immunoprecipitates with USP19. 3xflag-EWS-FLI1, 3xflag-EWSR1 or 3xflag-FLI1 were co-expressed with 3xmyc-USP19 in HEK293 cells for 48 h. After co-immunoprecipitation, lysates were analyzed by western blotting as indicated. (d) USP19 depletion increases EWSR1 monoubiquitination. A673 cells were transiently incubated with two different siRNAs against USP19 or a control siRNA for 24 h followed by co-expression of 3xflag-EWSR1 and HA-ubiquitin for 48 h. Ubiquitination of EWSR1 was analyzed by western blot analysis using anti-HA antibody. Full-length blots of all immunoprecipitates are presented in Supplementary Fig. S6.